Protocolos

Cuánto, cuántos y cada cuánto: ensayos e intervalos

Dos ensayos al día pueden ser casi tan buenos como cuatro. Esto es lo que más dicen las notas.

¿Cuánto debe durar un ensayo? ¿Cuántos al día? ¿Cuánto tiempo entre ellos? Las notas originales dan respuestas prácticas, que difieren un poco entre ratas y ratones. Aquí están una junto a otra, cada una con sus palabras originales.

Para ratas

Las notas originales de aprendizaje sugieren sacar a la rata si no ha encontrado la plataforma tras unos dos minutos, y dejarla en la plataforma unos 15 segundos para que mire alrededor. Las pruebas de sondeo duran de 1 a 2 minutos. Léelas completas en aprender a encontrar la plataforma y las ratas pueden hacer trampa.

Para ratones

Del watermaze.org original (1998-2013)

Tiempo e intervalos de nado

1. We use 2 mins in a 2m pool with a 15 cm platform. If your pool is as small as 1m, I would swim them for 1 min, & when they fail to find the platform, guide them to it so they climb on. Ideally you leave them there for 10 seconds, so they assess the surroundings. In practice they will make a leap for it if you let them. So as they climb on, step well-back & try to ensure they stay on for 10 seconds. If they immediately take to the water & keep swimming, intervene again to guide them back. They have to learn that rescue is dependent on staying on that platform.
2. If they leap for you before you retreat, you may as well catch them, as at least they have got the idea. You should find that on the first day your mice mostly fail to find the platform. Then on subsequent dates their efforts are greeted with increasing success. If you leave them too long at the beginning, swimming around without success, they will associate the swim with failure & panic.
3. We find that mice given 2 trials per day learn nearly as quickly as 4 times a day. If you are worried about the stamina of elderly mice
, cut back to 2 per day. Our mice look forward to their swim (they rattle the tops of their cages when my postdoc comes to collect them). We suspect they possibly enjoy it too much, and extend the swim-time because the alternative is to go back in a boring cage. Then we tried lowering the temperature of the pool by 2 deg, and they went on strike ! What I was leading-up to is that if the mice are not panicking, I suspect the swim is not traumatic, and they enjoy doing laps of the pool.
4. Intervals between swims is important. We use 30 mins, as being a good way to reinforce learning.

Texto original (en inglés): Dr. Roger J Morris, 1998, con notas del editor de Richard Baker. Copyright (C) 1998 RJ Morris & HVS Image. Reproducido sin cambios. Copyright Richard Baker© 2013. All Rights Reserved.

Los asteriscos de las notas sobre ratones están en el original. No hemos averiguado a qué se referían.

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